nir laser and camera system Search Results


personal lfr plus laser camera  (Lasergraphics Inc)
90
Lasergraphics Inc personal lfr plus laser camera
Personal Lfr Plus Laser Camera, supplied by Lasergraphics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+laser+and+camera+system/personal+lfr+plus+laser+camera/pmc02139782-126-12-19
Average 90 stars, based on 1 article reviews
personal lfr plus laser camera - by Bioz Stars, 2026-10
90/100 stars
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laser beam profiling camera  (Ophir Spiricon LLC)
90
Ophir Spiricon LLC laser beam profiling camera
Laser Beam Profiling Camera, supplied by Ophir Spiricon LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+laser+and+camera+system/laser+beam+profiler/pmc03960975-85-12-16
Average 90 stars, based on 1 article reviews
laser beam profiling camera - by Bioz Stars, 2026-10
90/100 stars
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atom probe microscope cameca leap 4000x hr  (CAMECA Inc)
90
CAMECA Inc atom probe microscope cameca leap 4000x hr
Atom Probe Microscope Cameca Leap 4000x Hr, supplied by CAMECA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+laser+and+camera+system/leap+4000x+hr/pmc09902532-159-11-10
Average 90 stars, based on 1 article reviews
atom probe microscope cameca leap 4000x hr - by Bioz Stars, 2026-10
90/100 stars
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laser-assisted wide-angle tomographic atom probe  (CAMECA Inc)
90
CAMECA Inc laser-assisted wide-angle tomographic atom probe
Laser Assisted Wide Angle Tomographic Atom Probe, supplied by CAMECA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+laser+and+camera+system/tomographic+atom+probe++tap++detection+system/10__1186_slash_1556___276x___8___39-67-14-8
Average 90 stars, based on 1 article reviews
laser-assisted wide-angle tomographic atom probe - by Bioz Stars, 2026-10
90/100 stars
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laser scanning confocal microscope  (Yokogawa Electric)
99
Yokogawa Electric laser scanning confocal microscope
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Laser Scanning Confocal Microscope, supplied by Yokogawa Electric, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+laser+and+camera+system/CSU-X1/pmc09016749-133-13-22
Average 99 stars, based on 1 article reviews
laser scanning confocal microscope - by Bioz Stars, 2026-10
99/100 stars
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pulsed laser apt instrument  (CAMECA Inc)
90
CAMECA Inc pulsed laser apt instrument
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Pulsed Laser Apt Instrument, supplied by CAMECA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+laser+and+camera+system/pulsed+laser+apt+instrument/pmc05321712-40-1-7
Average 90 stars, based on 1 article reviews
pulsed laser apt instrument - by Bioz Stars, 2026-10
90/100 stars
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orca-fusion cmos camera  (Hamamatsu)
90
Hamamatsu orca-fusion cmos camera
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Orca Fusion Cmos Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+laser+and+camera+system/orca+fusion+digital+cmos+camera/pmc10079432-131-14-13
Average 90 stars, based on 1 article reviews
orca-fusion cmos camera - by Bioz Stars, 2026-10
90/100 stars
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spectralis hra + oct laser scanning camera  (heidelberg engineering)
90
heidelberg engineering spectralis hra + oct laser scanning camera
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Spectralis Hra + Oct Laser Scanning Camera, supplied by heidelberg engineering, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+laser+and+camera+system/spectralis+oct/pmc09812261-136-17-30
Average 90 stars, based on 1 article reviews
spectralis hra + oct laser scanning camera - by Bioz Stars, 2026-10
90/100 stars
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imagem emccd camera  (Hamamatsu)
90
Hamamatsu imagem emccd camera
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Imagem Emccd Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+laser+and+camera+system/imagem+emccd+camera/pmc06143344-166-25-28
Average 90 stars, based on 1 article reviews
imagem emccd camera - by Bioz Stars, 2026-10
90/100 stars
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progres c10 plus digital camera  (JENOPTIK Inc)
90
JENOPTIK Inc progres c10 plus digital camera
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Progres C10 Plus Digital Camera, supplied by JENOPTIK Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+laser+and+camera+system/progres+c3+camera/pm23931896-331-12-18
Average 90 stars, based on 1 article reviews
progres c10 plus digital camera - by Bioz Stars, 2026-10
90/100 stars
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orca er camera  (Hamamatsu)
90
Hamamatsu orca er camera
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Orca Er Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+laser+and+camera+system/em+ccd+camera/pmc03627647-268-10-16
Average 90 stars, based on 1 article reviews
orca er camera - by Bioz Stars, 2026-10
90/100 stars
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orca-r2 camera  (Hamamatsu)
90
Hamamatsu orca-r2 camera
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Orca R2 Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+laser+and+camera+system/orca+er+camera/pmc04423121-419-23-25
Average 90 stars, based on 1 article reviews
orca-r2 camera - by Bioz Stars, 2026-10
90/100 stars
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Image Search Results


A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal microscope images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.

Journal: Analytical chemistry

Article Title: A Novel Omniphobic Platform for Multicellular Spheroid Generation, Drug Screening, and On-Plate Analysis

doi: 10.1021/acs.analchem.1c01326

Figure Lengend Snippet: A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal microscope images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.

Article Snippet: The stained cells were carefully transferred to glass coverslips and imaged using a laser scanning confocal microscope (Olympus IX81, equipped with a Yokogawa CSU-X1 confocal scanning laser unit, Andor iXon x3 CCD camera, and Metamorph 7.8 software), with constant gain and exposure settings between samples.

Techniques: Generated, Two Tailed Test, Fluorescence, Control, Cell Culture, Microscopy, Labeling